Antioxidant activity of cultivated mushrooms (Pleurotus Florida, Pleurotus Sajur-Cajo, and Lentinus Edodes) in Sabah

Choong, Jian Ming (2010) Antioxidant activity of cultivated mushrooms (Pleurotus Florida, Pleurotus Sajur-Cajo, and Lentinus Edodes) in Sabah. Universiti Malaysia Sabah. (Unpublished)


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Objective of this study is to determine antioxidant activity of various species of mushroom extracted using different solvents. Besides, it also evaluate the ability of SPE to elevate the antioxidant activity of mushroom extract. Cultivated mushrooms in Sabah including Pleurotus florida, Pleurotus sajur-cajo, and Lentinus edodes were analyzed for their antioxidant activity as measured by DPPH radical scavenging activity assay, Beta-carotene bleaching inhibition assay, ABTS Trolox equivalent assay, FRAP ion reduction assay and ABTS Trolox Equivalent assay. L. edodes water extract with the yield of 26.16%, 2.55mg/ml of EC value of DPPH Scavenging activity, 5.46mg/ml of ECso value for B-Carotene Bleaching Inhibition, 253.13 umol Trolox/mg extract and 0.2093 mmol Fe (II)/mg extract. Among the methanolic and water extracts of the mushrooms, L.edodes water extract was selected to underwent solid phase extraction (SPE) due to it consistent antioxidant activity for the four antioxidant assays. After SPE, three fractions were obtained and they were underwent the four antioxidant activity assays. From the three fractions, the 5% methanol of the water extract of L.edodes, shown the best antioxidant activity among other fractions with, 350.42 umol Trolox/mg extract and 0.7947 mmol Fe(II)/mg extrac and the antioxidant activity of the fraction is higher than the crude water extract of L. edodes.

Item Type: Academic Exercise
Keyword: Mushrooms, Pleurotus florida, Pleurotus sajur-cajo, Lentinus edodes, Antioxidant activity, Sabah
Subjects: Q Science > QK Botany > QK1-989 Botany > QK504-(638) Cryptogams
Department: SCHOOL > School of Food Science and Nutrition
Depositing User: ADMIN ADMIN
Date Deposited: 16 Nov 2011 17:09
Last Modified: 12 Oct 2017 14:18

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