Separation of phytosterols using chitosan from Penaeus Monodon

Chee, Chooi Yee (2004) Separation of phytosterols using chitosan from Penaeus Monodon. Universiti Malaysia Sabah. (Unpublished)


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Separation of phytosterol using chitosan from shell of shrimp (Penaeus monodon) has been investigated. The separation ability of phytosterol by chitosan is correlated with the binding ability of chitosan to phytosterol. Binding ability of chitosan with phytosterol is expressed in Binding Factor (BF). The first step in qualification of the binding property is the establishment of a standard calibration curve from interaction between chitosan with standard cholesterol and interaction between standard chitosan and standard p-sitosterol, commonly occurring in plant sterol. Analysis of the interaction involving chitosan with cholesterol is essential for comparison with phytosterol binding. The range of BF values obtained from the BF versus [P] graph for the interaction of chitosan with standard p-sitosterol range from 3.78 to 4.84 while the range of BF value is from 4.21 to 5.82 for the interaction involving chitosan from shrimp shell with p-sitosterol. The increase in BF values indicate a positive interaction but negative binding cooperactivity is postulated when [CP]/ [P] versus [P] is plotted. However, utilizing the BF versus [P] graph for the interaction of shrimp shell chitosan with standard p-sitosterol as a standard calibration curve, chitosan from shrimp shell is able to bind to approximately 4mgmrl of p-sitosterol in soy sample.

Item Type: Academic Exercise
Keyword: phytosterol, chitosan , shrimp (Penaeus monodon), Binding Factor (BF),
Subjects: Q Science > Q Science (General)
Department: SCHOOL > School of Science and Technology
Date Deposited: 22 Oct 2013 13:06
Last Modified: 17 Oct 2017 12:25

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