An investigation of bioactive constituents in tropical sponges

Toh, Han Loong (2007) An investigation of bioactive constituents in tropical sponges. Universiti Malaysia Sabah. (Unpublished)


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The common occurrence of biologically active natural products in sponges reflects an important ecological adaptation as chemical defense. Some of these chemicals have been found to have beneficial pharmaceutical effect for human. As such, the purpose of this research is to investigate the potential of tropical sponges for bioactive properties. Crude extract for 3 tropical sponges obtained using extraction while TLC and PTLC Bioassay were used to screen the crude extracts for bioactive properties. Further separation was done using PTLC to obtain major compound. Bioactive compounds in crude extract is then characterise using Rf values, UV illumination and TLC spray reagents. Results indicate that species 3 produced the highest crude extract yield of 1.1315 percent followed by species 2 with 0.1944 percent and species 1-lowest with 0.0843 percent. Most of the compounds in species 1 are non polar whereas compounds in species 2 and 3 are of high polarity. Hexane crude extract for species 1 and methanol crude extract for species 3 shown activities against marine bacteria Clostridium cellabioparum, Clostridium novyi and Vibrio parahaemolyticus. TLC characterisation conclude that bioactive compound in species 1 is probably sterol with Rf value of 0.87 in HE (3:1 v/v) solvent while bioactive compound in species 3 could be glicolipid with Rf value of 0.24 in CMW (6.5:3.5:0.4 v/v/v) solvent.

Item Type: Academic Exercise
Keyword: pharmaceutical, bioactive, UV illumination, TLC spray reagent, Hexane crude extract, natural product, polarity
Subjects: Q Science > QH Natural history > QH301-705.5 Biology (General)
Department: SCHOOL > School of Science and Technology
Date Deposited: 25 Mar 2014 14:24
Last Modified: 13 Oct 2017 09:25

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