Molecular phylogenetics on genus pronephrium (thelypteridaceae) in Sabah using rbcL gene sequences

Liau, Jacqueline Shuk Yee (2008) Molecular phylogenetics on genus pronephrium (thelypteridaceae) in Sabah using rbcL gene sequences. University Malaysia Sabah. (Unpublished)


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Gene rbcL which found in genome chloroplast is used in this study. Nucleotide sequences of three samples of Pronephrium (P. nitidum, P. repandum, and P. asperum) were obtained. Apart from that, nucleotide sequences of three species of Thelypteris and one species from Aglaomorpha (outgroup), including T. ovata, T. longissima, T. simplex and A. heraclea were obtained from GenBank were used in the phylogenetic tree construction using Paup Version 4.0b10 for Macintosh. Maximum Parsimony (MP) and Maximum Likelihood (ML) analyses were performed in this study. Trees obtained from MP and ML analyses showed two different topologies. P. nitidum and P. repandum suggested as a sister taxa in both analyses and supported by a high bootstrap (BS) value of 100%. However, P. asperum was clustered into Thelypteris clade with a weak BS value of 59% in the ML analysis. Hence, conclusion can be made that there is a weak relationship of Pronephrium in relation to Thelypteris in Thelypteridaceae. However, extensive study should be done for both the genera to provide a clear- cut classification between these two genera. It is also suggested that morphological data such as the spore character should be used to provide a more consistent and reliable information for this study.

Item Type: Academic Exercise
Keyword: gene rbcL, genome chloroplast, phylogenetic, Paup Version 4.0b10, Maximum Parsimony (MP), Maximum Likelihood (ML)
Subjects: Q Science > QH Natural history > QH301-705.5 Biology (General)
Department: SCHOOL > School of Science and Technology
Date Deposited: 03 Jun 2014 15:13
Last Modified: 13 Oct 2017 12:28

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