Extraction and purification of saxitoxin-binding protein from marine resource

Muhd Faizul Helmi Ahamad Hasmi (2007) Extraction and purification of saxitoxin-binding protein from marine resource. University Malaysia Sabah. (Unpublished)


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This study aims to extract and partially purify the saxitoxin-binding protein from the plasma protein of Arothron reticularis, puffer fish. The fish samples were collected along Pantai Tanjung Lipat and Sungai Likas. A total of 20 mt of plasma was collected after centrifugation of 39.6 ml of blood withdrawn from 12 fresh fish samples. This plasma contained 811.2 mg of protein. The amount of protein decreased drastically to 184.79 mg after the addition of solid ammonium sulfate to 50 and 70 percent of saturations. Next, the purification using Uno Q1 anion exchange column chromatography with 50 mM Tris HCI (PH 7.4) as the running buffer and 0.25 M NaCl as the elution buffer separated the proteins into five fractions. The highest fraction peak collected is in the F5 fraction where the absorbance was 0.2382 and the lowest peak is in the F3 fraction, with absorbance of 0.0093. The other three fractions are FI with 0.0317, F2 with 0.0174 and finally the F4 with absorbance of 0.0699. After further separation of the total protein sample left, the same fractions from each separation were pooled together and the concentrations of each fraction were determined. Finally, FI shows the presence of 4.423 mg of protein, F2 with 3.907 mg, F3 with 2.268 mg, F4 with 9.421mg and F5 with 27.568 mg.

Item Type: Academic Exercise
Keyword: saxitoxin-binding, protein, extraction, purification, marine resourse
Subjects: Q Science > QH Natural history > QH301-705.5 Biology (General)
Department: SCHOOL > School of Science and Technology
Date Deposited: 09 Jun 2014 14:01
Last Modified: 13 Oct 2017 12:32
URI: https://eprints.ums.edu.my/id/eprint/9118

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