Selyvester @ Julkarnain Kundian (2006) Purification of chitinase produced by Streptomyces BRI 36. Universiti Malaysia Sabah. (Unpublished)
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Abstract
Spore suspension of Streptomyces BRI 36 was inoculated in 100 ml of enzyme production media in two batches. The synthesis of an extracellular Chitinase by Streptomyces BRI 36 was induced by using colloidal chitin as its sole carbon source. Chitinase was isolated from the culture filtrate and purified by acetone precipitation, Sephadex G-25, Sephadex G-50 and Sephadex 0-100 gel filtration. Enzyme assay was done by using colloidal chitin stained with Remazol Brilliant Blue® as substrate. Final yield was about 21 % for batch I and 9% for batch 2. Total protein was estimated by using modified Lowry method and given estimation of 5.15 mg for batch 1 and 1.88 mg for batch 2.
| Item Type: | Academic Exercise |
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| Keyword: | Streptomyces BRI 36, enzyme, colloidal chitin, culture filtrate, protein |
| Subjects: | Q Science > QH Natural history > QH301-705.5 Biology (General) |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 27 Jan 2015 13:52 |
| Last Modified: | 24 Oct 2017 13:51 |
| URI: | https://eprints.ums.edu.my/id/eprint/10406 |
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