Joko Sulistyo and Rita Dwi Rahayu and Rini Handayani (2008) Sinthesis of polyphenol glycoside by using maltodextrin through enzymatic transglycosilation reaction of bacterial strain. In: Proceeding of The International Seminar on Chemistry 2008, 30-31 October 2008, Jatinangor, Indonesia.
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Abstract
Indigenous microbial strains produced a glycosyl transfer enzyme (CGTase EC. 2.4.1.19) of Bacillus licheniformis BL-1 in an acetate buffer pH 5.5 at 45˚C for 24h, yielding polyphenol glycoside as transfer product in the present of maltodextrins those were hydrolysed from different sources of starch as a donor-substrate and polyphenols from rhyzomes as an acceptor those were considered to have interesting biological activity such as antimicrobial and antioxidant. It was found that polyphenol-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200ppm on the growth of Bacillus substilis, Escherichia coli and Saccharomyces cerevisiae, however no effected when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains. We suggested that the polyphenol glycoside possessed antioxidative activity from the view point of retaining the bleaching value of β-carotene. The result showed that polyphenol glycoside was found to be higher than of arbutin as authentic glycoside and polyphenol aglycone, however, it was lower than of BHT (butylated hydroxy toluene), a commercial antioxidant product. This result suggested that polyphenol glycoside possessed considerable antioxidative activity by chelating free radicals, and this effect seem to be available for practical use.
| Item Type: | Conference or Workshop Item (UNSPECIFIED) |
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| Keyword: | CGT-ase, Aspergillus oryzae, maltodextrin, rhizome, polyphenol, transglycosylation, antimicrobial, antioxidant |
| Subjects: | Q Science > QR Microbiology |
| Department: | FACULTY > Faculty of Food Science and Nutrition |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 11 May 2016 13:57 |
| Last Modified: | 10 Nov 2017 10:13 |
| URI: | https://eprints.ums.edu.my/id/eprint/12625 |
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