Kelvin Henry (2009) Analysis of analgesics and their metabolites in human urine using capillary electrophoresis. Universiti Malaysia Sabah. (Unpublished)
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Abstract
Acetylsalicylic acid (Aspirin) is rapidly metabolized to salicylic acid (salicylate) and other compounds, including gentisic acid and salicyluric acid. Monitoring of salicylate and its metabolites is of toxicological, pharmacological and biomedical interest. The separation of acetylsalicylic acid and its metabolites has been carried out by using non-aqueous capillary electrophoresis system with reversed electroosmotic flow. The flow is reversed by adding polycation hexadimethrine bromide and using a negative voltage. This method provides a fast and effective separation of the acetylsalicylic acid and its metabolites. The separation of all urine samples was performed on a fused-silica capillary (59.3 cm x 50, µm ID; 49.3 cm to detector). The result was favorable when using 214 nm detection, temperature 25°C and voltage -30 kV. By comparing blank urine and urine samples taken after administration of 500 mg aspirin tablet shows that there were changes in number of peaks appear in the electropherogram of urine sample taken after administration of 500 mg aspirin. This new peaks can be considered as the metabolites of acetylsalicylic acid. Comparison of electropherogram of urine samples 2 and 6 hour after administration of aspirin shows that there were changes in the height of the peaks between the samples.
| Item Type: | Academic Exercise |
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| Keyword: | aspirin, salicylic acid, gentisic acid, salicyluric acid, toxicological, pharmacological, biomedical interest |
| Subjects: | Q Science > QD Chemistry |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 15 Jul 2016 16:27 |
| Last Modified: | 20 Nov 2017 11:38 |
| URI: | https://eprints.ums.edu.my/id/eprint/13612 |
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