Mailin Misson, and Du, Xin and Jin, Bo and Zhang, Hu (2016) Dendrimer-like nanoparticles based beta-galactosidase assembly for enhancing its selectivity toward transgalactosylation. Enzyme and Microbial Technology, 84. pp. 68-77. ISSN 0141-0229
Full text not available from this repository.Abstract
Functional nanomaterials have been pursued to assemble nanobiocatalysts since they can provide unique hierarchical nanostructures and localized nanoenvironments for enhancing enzyme specificity, stability and selectivity. Functionalized dendrimer-like hierarchically porous silica nanoparticles (HPSNs) was fabricated for assembling β-galactosidase nanobiocatalysts for bioconversion of lactose to galacto-oligosaccharides (GOS). The nanocarrier was functionalized with amino (NH2) and carboxyl (COOH) groups to facilitate enzyme binding, benchmarking with non-functionalized HPSNs. Successful conjugation of the functional groups was confirmed by FTIR, TGA and zeta potential analysis. HPSNs-NH2 showed 1.8-fold and 1.1-fold higher β-galactosidase adsorption than HPSNs-COOH and HPSNs carriers, respectively, with the highest enzyme adsorption capacity of 328 mg/g nanocarrier at an initial enzyme concentration of 8 mg/ml. The HPSNs-NH2 and β-galactosidase assembly (HPSNs-NH2-Gal) demonstrated to maintain the highest activity at all tested enzyme concentrations and exhibited activity up to 10 continuous cycles. Importantly, HPSNs-NH2-Gal was simply recycled through centrifugation, overcoming the challenging problems of separating the nanocarrier from the reaction medium. HPSNs-NH2-Gal had distinguished catalytic reaction profiles by favoring transgalactosylation, enhancing GOS production of up to 122 g/l in comparison with 56 g/l by free β-galactosidase. Furthermore, it generated up to 46 g/l GOS at a lower initial lactose concentration while the free counterpart had negligible GOS production as hydrolysis was overwhelmingly dominant in the reaction system. Our research findings show the amino-functionalized HPSNs can selectively promote the enzyme activity of β-galactosidase for transgalactosylation, which is beneficial for GOS production.
| Item Type: | Article |
|---|---|
| Keyword: | Nanobiocatalysis, β-Galactosidase, Dendrimer-like hierarchical pores silica nanoparticles, Galacto-oligosaccharides |
| Subjects: | Q Science > QR Microbiology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 03 Aug 2016 14:12 |
| Last Modified: | 03 Aug 2016 14:12 |
| URI: | https://eprints.ums.edu.my/id/eprint/13741 |
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