Katia Ferreira Rodrigues and S. V. Kumar (2009) Isolation and characterization of 24 microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid. Conservation Genetics, 10 (1). pp. 127-130. ISSN 1566-0621
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Isolation_and_characterization_of_24_microsatellite_loci_in_Paphiopedilum_rothschildianum,_an_endangered_slipper_orchid.pdf Download (128kB) | Preview |
Abstract
This paper describes the characterization of 24 polymorphic microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid endemic to Sabah, Malaysia. A total of 36 clones containing microsatellite repeat motifs yielded 41 dinucleotide, 20 tetranucleotide and 30 cryptic simple repeat sequences. Thirty microsatellite loci were randomly selected to characterize the population, of which 24 were found to be polymorphic. The expected heterozygosity ranged from 0.0813 to 0.7279 (mean 0.4533) and the observed heterozygosity ranged from 0 to 1 (mean 0.3800). The results indicated that there was limited migration between the three subpopulations and a significant degree of genetic differentiation (FST = 0.5098) between populations implying that gene flow has been disrupted as a result of habitat fragmentation. The molecular markers have a potential application in population management, CITES enforcement and the forensic detection of specimens in international trade. © 2008 Springer Science+Business Media B.V.
| Item Type: | Article |
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| Keyword: | 5′ anchored PCR, CITES, Heterozygosity, Microsatellite, Paphiopedilum rothschildianum |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 22 Mar 2011 14:08 |
| Last Modified: | 19 Oct 2017 14:45 |
| URI: | https://eprints.ums.edu.my/id/eprint/1531 |
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