Nur Syafiqah Abdullah Hisham (2013) Phytochemical screening and antimicrobial activity of plant extracts of semalu plant (mimosa pudica l.). Universiti Malaysia Sabah. (Unpublished)
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Abstract
A study was conducted on different parts of Mimosa pudica L which are leaves, flower and stem that collected around UMS, Sandakan campus. The purpose of this study is to screen phychemical content and investigate antimicrobial activities on different plant parts of M. pudica. The study was conducted in the School of Sustainable Agriculture laboratory and starting in April 2012 until October 2012. In this study, preliminary phytochemical by qualitative test and antimicrobial activity towards different parts of M. pudica was conducted by using ethanol as solvent extraction. Phytochemical screening result was obtained from visual observation revealed the presence of glycosides, saponins, flavonoids, tannins and phenolic compound. The antimicrobial activity of different plant parts were investigated by disc diffusion method against bacterial pathogen E. coli, S. aureus, S. enteritis, C freundii. The zone of inhibition of crude extracts was compared with standard antibiotic streptomycin (Smcg/disc) and diameter of inhibition zone was observed after 24 hours incubation period. According to this study, in terms of bacterial activity (zone of inhibition) as whole showed a significant difference mean values (p<0.05) for all crude extract. Among the three different parts, crude extracts of leaves showed higher inhibition zone of 9.8+2.98mm. This study proceeds with minimum concentration for inhibitory zone and was found that lowest concentration of leaves extract at 5mg/ml against E. co"; while S. aureus and S. enteritis showed inhibition zone at the lowest concentration of crude extract at 10mg/ml and bacteria C freundii showed lowest inhibition zone at concentration 40mg/ml. The lower concentration of the crude extract that can inhibit bacteria, the higher the potential of the plant can be used in medical field hence through this study the abilities of M. pudica extracts inhibit bacteria at low concentrations with the higher amount of crude extract produced prove that this plant has potential as an effective antimicrobial agent.
| Item Type: | Academic Exercise |
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| Keyword: | Mimosa pudica, antimicrobial activity, phytochemical screening, disc diffusion method, minimum inhibitory concentration. |
| Subjects: | S Agriculture > SB Plant culture |
| Department: | FACULTY > Faculty of Sustainable Agriculture |
| Depositing User: | MDM FAUZIAH MATSIN |
| Date Deposited: | 05 Jul 2017 09:59 |
| Last Modified: | 13 Oct 2017 15:43 |
| URI: | https://eprints.ums.edu.my/id/eprint/17085 |
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