Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization

Mailin Misson and Bo, Jin and Binghui, Chen and Hu, Zhang (2015) Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization. Bioprocess and Biosystems Engineering, 38 (10). pp. 1915-1923. ISSN 1615-7605

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URL: https://doi.org/10.1007/s00449-015-1432-5

Abstract

A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications.

Item Type:	Article
Keyword:	Nanobiocatalysts, Galacto-oligosaccharides, β-galactosidase, Polymer nanofibers, Bioconversion
Subjects:	T Technology > TA Engineering (General). Civil engineering (General)
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	MUNIRA BINTI MARASAN -
Date Deposited:	27 Mar 2018 15:25
Last Modified:	09 Dec 2020 10:50
URI:	https://eprints.ums.edu.my/id/eprint/19596

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