Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Mailin Misson and Bo Jin and Sheng Dai and Hu Zhang (2020) Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides. Catalysts, 10 (81). pp. 1-12.

Text
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides.pdf
Restricted to Registered users only
Download (2MB)

URL: https://doi.org/doi:10.3390/catal10010081

Abstract

Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modiﬁed electrospun polystyrene nanoﬁbers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and ﬂuorescence analysis of ﬂuorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at are sidence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanoﬁber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

Item Type:	Article
Keyword:	β-galactosidase, polymer nanoﬁbers, nanobiocatalyst, interfacial characterization, interfacial reaction, conformational change
Subjects:	Q Science > Q Science (General)
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	20 Feb 2020 11:11
Last Modified:	08 Apr 2021 22:49
URI:	https://eprints.ums.edu.my/id/eprint/24984

Actions (login required)

View Item