Yuki Midorikawa and Tomohito Shimizu and Tetsuya Sanda and Katsuyuki Hamasaki and Shigeki Dan and Mohammad Tamrin Mohamad Lal and Goshi Kato and Motohiko Sano (2020) Characterization of Aquimarina hainanensis isolated from diseased mud crab Scylla serrata larvae in a hatchery. Journal of Fish Diseases. ISSN 1365-2761
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Characterization of Aquimarina hainanensis isolated from diseased mud crab Scylla serrata larvae in a hatchery.pdf Download (206kB) | Preview |
Abstract
Mass mortality due to necrosis signs occurred in hatchery‐reared zoea stage larvae of the mud crab Scylla serrata in Okinawa, Japan, and a causative bacterium was isolated. In this study, we identified and characterized the bacterium by genome analysis, biochemical properties and pathogenicity. The bacterium was a Gram‐negative, non‐motile, long rod, forming yellow colonies on a marine agar plate. It grew at 20–33°C (not at 37°C) and degraded chitin and gelatin. Phylogenetic analysis of the 16S rRNA gene sequence identified the bacterium as Aquimarina hainanensis. Genome sequence data obtained from Illumina MiSeq generated 29 contigs with 3.56 Mbp in total length and a G + C content of 32.5%. The predicted 16 chitinase genes, as putative virulence factors, had certain homologies with those of genus Aquimarina. Experimental infection with the bacterium conducted on larvae of four crustacean species, brine shrimp Artemia franciscana, freshwater shrimp Caridina multidentata, swimming crab Portunus trituberculatus and mud crab S. serrata, revealed that this bacterium was highly virulent to these species. The present study suggests that the bacterium caused mass mortality in mud crab seed production was A. hainanensis and can be widely pathogenic to crustaceans.
| Item Type: | Article |
|---|---|
| Keyword: | Larvae , mud crab Scylla Serrata , Japan |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | NORAINI LABUK - |
| Date Deposited: | 16 Apr 2020 11:51 |
| Last Modified: | 16 Apr 2020 11:51 |
| URI: | https://eprints.ums.edu.my/id/eprint/25308 |
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