Vun, Yee Thien and Thau, Wilson Lym Yong and Ann Anton and Grace Joy Wei Lie Chin (2020) A multiplex PCR method for rapid identification of commercially important seaweeds Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum (Rhodophyta, Solieriaceae). Regional Studies in Marine Science, 40.
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A multiplex PCR method for rapid identification of commercially important seaweeds Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum (Rhodophyta, Solieriaceae).pdf Download (47kB) | Preview |
Abstract
Taxonomic clarification of Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum based on standard DNA barcodes has proven to be useful as the phenotypic plasticity of the species can confound traditional taxonomic approaches. Molecular characterization of each isolate is time-consuming and a painstaking task as multiple runs of PCR amplification needs to be carried out and the identity of the isolates is only known after sequencing of the PCR amplicons. In this study, we have developed a simple, efficient and reproducible one-step polymerase chain reaction (PCR) based method for the identification of the three eucheumatoid species, K. alvarezii, K. striatus and E. denticulatum. Based on the nuclear ribosomal internal transcribed spacer (ITS) sequence, all the available genetic polymorphisms were reviewed and three primers were designed. The combination of these primers in the same PCR reaction results in a specific amplicon of distinct size ranging from approximately 140-400 bp to be produced for each of the three red algal species. The proposed method is found to be an effective and practical tool to aid in the selection of the desired seaweed seedlings even before they are cultivated and also the identification of commercial cultivars accurately.
| Item Type: | Article |
|---|---|
| Keyword: | Eucheumatoid, Internal transcribed spacer, Molecular marker, Polymerase chain reaction, Species identification |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 10 Nov 2020 14:03 |
| Last Modified: | 10 Nov 2020 14:03 |
| URI: | https://eprints.ums.edu.my/id/eprint/26292 |
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