Chong, Vivian Ai Ping (2017) Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles. Masters thesis, Universiti Malaysia Sabah.
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Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles.pdf Restricted to Registered users only Download (3MB) |
Abstract
Extracts of two edible mushrooms, Pleurotus sajor caju (commercial) and Schizophyllum commune (wild) were used to study the chemical compositions and the potency to use as feed additive on the immune response in Asian seabass, Lates calcarifer juveniles. Four types of solvents namely aqueous, ethanol, methanol and acetone were used in mushrooms extraction process to assess total polysaccharide, crude lipid, protein, amino acids, fatty acids, antioxidant activity and antimicrobial activity in mushrooms crude extracts. In feeding trial, one percent of aqueous P. sajor caju extract added diet (D1), one percent of aqueous S. commune extract added diet (D2) and commercial diet without mushroom extract (D0) were fed to L. calcarifer juveniles for 30 days. After 30 days, challenge assay was conducted by injecting each fish intraperitoneally with 108cfu/ml of V. harveyi bacterium suspension. Immune response was investigated by measuring antibody titre from blood serum and the survival rate (%) was observed for 10 days post-challenge. Total polysaccharide, crude lipid and protein composition were ranged from 2.07% to 22.08%, 0.01% to 0.32%, 5.07% to 17.14% and 13.34% to 25.48%, 0.10% to 0.65%, 6.25% to 10.70% in P. sajor caju and S. commune crude extract, respectively. P. sajor caju contained 19.04% to 20.34% of total AA and S. commune contained 20.29% to 31.62%. Aqueous extract has the highest amount of total EAA (8.95 ± 0.02%) in both mushrooms. DPPH scavenging activity obtained the highest antioxidant activity in 5mg/ml of S. commune and P. sajor caju extracts. P. sajor caju and S. commune aqueous extracts at 2mg/ml have shown the highest reducing power capability at 2.69 and 3.35 (700nm), respectively. The lowest EC50 (mg/ml) of in-vitro antioxidant assays were obtained in P. sajor caju (DPPH=1.47; reducing power=0.09) and S. commune (DPPH=1.52; reducing power=0.41) with significance of p <0.05 in aqueous extracts. Minimum inhibition concentration (MIC) values of V. harveyi, V. parahaemolyticus and V. anguillarum were <1.25mg/ml, <1.25mg/ml, 10mg/ml and <1.25mg/ml, 2.5mg/ml, 5mg/ml obtained in P. sajor caju and S. commune extracts, respectively. Fish fed in D1 and D2 have shown significance difference (p<0.05) in antibody titre compared to antibody titre obtained in D0 (control diet). D2 also demonstrated the highest survival of 90%, while fish fed with D1 and D0 recorded 55% and 60% of survival, respectively. The extract of S. commune has shown better chemical composition with aqueous extraction process. The highest survival of 90% with the development of antibody titre were also observed in S. commune extract inclusion diet. Extract of S. commune is the choice of option to boost immune system in Asian seabass juveniles.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | mushrooms, Pleurotus sajor caju (commercial) , Schizophyllum commune (wild) , Asian seabass, |
| Subjects: | Q Science > QK Botany |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | NORAINI LABUK - |
| Date Deposited: | 17 Dec 2020 16:36 |
| Last Modified: | 17 Dec 2020 16:36 |
| URI: | https://eprints.ums.edu.my/id/eprint/26499 |
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