Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey

Monjia Belleza Cosmas Mojulat (2018) Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey. UNSPECIFIED thesis, Universiti Malaysia Sabah.

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Abstract

Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. Based on analysis of 1 g of sample extracted with 100ml deionised water, the optimal extraction conditions for aqueous extraction were determined to be at 700C for 20 minutes where its TPC was 9.97+0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.64+0.01 mg QUE/mL (p<0.05) while its FRAP assay was amounted to 16.84+1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be 87.53% with its IC50 at 492.54+2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. The aforementioned tests and assays recorded higher antioxidant activity with increasing honey dosage (max 4tbsp.), where its TPC was 21.66+0.54 mg GAE/mL (p<0.05), while antioxidant activity based on FRAP assay yielded 24.02+0.87 mg TE/mL (p<0.05). Accordingly, radical scavenging activity based on DPPH assay was 98.20% and lower IC50 value compared to infusion at lower dosage, amounting at 408.02+4.98 mg TE/mL. Subsequently, gallic acid and quercetin isolated from freeze dried Carica papaya leaves extract was higher compared to freshly prepared sample. However, conversely, gallic acid and quercetin yielded from fresh “kelulut” honey and Carica papaya leaves extract infused with “kelulut” honey is higher in fresh sample, suggesting that preservation effect using freeze dry method differ depending on sample type and method of preparation. Future study can therefore be done to improve and develop standardised preparation of infused papaya leaves with honey, and subsequently releasing it for commercial usage.

Item Type:	Thesis (UNSPECIFIED)
Keyword:	Carica Papaya , Honey , Kelulut
Subjects:	S Agriculture > SF Animal culture
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	NORAINI LABUK -
Date Deposited:	27 May 2021 11:22
Last Modified:	27 May 2021 11:22
URI:	https://eprints.ums.edu.my/id/eprint/26572

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