Multi-step ft-ir spectroscopy analysis of the metabolite content in hibiscus sabdariffa var. UKMR-2 in response to soil media

Siti Aishah Mohd Ali and J. Latip (2020) Multi-step ft-ir spectroscopy analysis of the metabolite content in hibiscus sabdariffa var. UKMR-2 in response to soil media.

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Abstract

The present study analyze the differences in metabolite content in UKMR-2 calyces in response to different soil media formulation ratio treatment by applying multi-step infrared based fingerprinting. The cultivation using two different media ratio (top soil, organic matter and sand); SM1 with 2:1:1 v/v/v and SM2 with 2:1:2 v/v/v. Multi-step infrared based fingerprinting: one dimensional infrared (1D-IR), second derivative infrared (SD-IR) and two dimensional correlation infrared (2D-IR) spectroscopy were used. The 1D-IR spectrums revealed a similar absorption in the fingerprint zone (1900 - 650 cm-1). SD-IR spectrums clearly showed have more peaks with different shape, position and intensity in the fingerprint zone. 2D-IR synchronous correlation spectrum clearly distinguished the metabolite content in the UKMR-2 calyx extract in the range of 1000 – 650 cm-1 . Therefore, multi-step infrared based fingerprinting has the potential as one of the effective methods to discriminate extract samples with similar infrared fingerprint features.

Item Type:	Proceedings
Keyword:	Infrared based fingerprinting, UKMR-2, soil media, SD-IR, 2D-IR
Subjects:	Q Science > Q Science (General) Q Science > QK Botany
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	09 Jun 2021 11:09
Last Modified:	09 Jun 2021 11:09
URI:	https://eprints.ums.edu.my/id/eprint/26960

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