Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)

Mohd S Aidil and Mohd Khalizan Sabullah and Mohd Izuan Effendi Halmi and Mohd Rosni Sulaiman and Mohd Shukri Abd Shukor and Mohd Y. Shukor and Noor Azmi Shaharuddin and Mohd. Arif Syed and Amir Syahir (2013) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878). Fresenius Environmental Bulletin, 22 (12). pp. 3572-3576. ISSN 1018-4619

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URL: https://www.researchgate.net/publication/280056425...

Abstract

Acetylcholinesterase (AChE) is generally used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from silver catfish brain (Pangasius sp.), wasted from aquaculture industry, as an inhibitive assay for heavy metals. We discovered that the AChE was completely inhibited by Cr6+, Ag2+, Cd2+, Hg2+, Cu2+, Zn2+ and Pb2+ during an initial screening. When testing at various concentrations, the above heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for Hg2+, Cu2+, Ag2+, Cr6+, Cd2+, Pb2+, and Zn2+ were 0.071, 0.088, 0.088, 0.87, 0.913, 1.1, and 1.572 mg/L, respectively. The Limit of Detection (LOD) and Limit of Quantification (LOQ) values of the heavy metals are within the range of the Maximum Permissibility Limit (MPL) allowed by the Malaysian Department of Environment (DOE) for Class-III rivers. The IC50 values for these heavy metals are comparable, and some are lower than those of the currently known assays, such as immobilised urease, Daphnia magna, MicrotoxTM, rainbow trout, papain and bromelain assays. Field trial works on an industrial drainage site had shown that the developed assay is applicable in detecting heavy metals in the water. The assay can be carried out in less than 30 min under ambient temperature.

Item Type:	Article
Keyword:	Pangasius Sp., Acetylcholinesterase (AChE) , Heavy Metals , Inhibitive Assay
Subjects:	Q Science > QH Natural history > QH301-705.5 Biology (General) S Agriculture > SH Aquaculture. Fisheries. Angling
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	29 Jun 2021 08:47
Last Modified:	29 Jun 2021 08:47
URI:	https://eprints.ums.edu.my/id/eprint/27486

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