An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection

Siti Aishah Muhammad Khalidi and Mohd Khalizan Sabullah and Suraya Abd. Sani and Mohd Yunus Shukor and Siti Aqlima Ahmad and Nur Adeela Yasid and ‘Izazy Nur Mohd Jaafar and Gunasekaran Baskaran (2019) An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection. In: 3 rd International Conference on Molecular Biology and Biotechnology 2019, 24-25 April 2019, UCSI University Kuala Lumpur Campus.

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Abstract

Introduction: Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolve compound in any types of water sources especially river. Methods: This method can be applied as a preliminary screening to determine the contamination level of the river in a short of time as well as low cost and easy to operate. The aim of this study to assess the use of acetylcholinesterase source from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Results: Purified acetylcholinesterase was exposed with a different type of metal ion, and mercury show the highest inhibition which capable of lowering the enzyme activity to 0.018U followed by copper (1.280U), chromium (4.675U) and zinc (4.803U) at 10 ppm. Metal ions show exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order of copper, chromium, zinc and mercury at 1.329, 0.687, 0.595 and 0.004 ppm, respectively. Field trial works exhibited that the enzyme was applicable in sensing heavy metals pollution from the river which closes to the industrial and agricultural sites at near real time and verified using ICP-OES. Conclusion: This study proves the potential use of acetylcholinesterase source from M. albus as a biomonitoring tool to evaluate the contamination level of the river.

Item Type:	Conference or Workshop Item (Paper)
Keyword:	Pollution , Biosensor , Monopterus albus , Cholinesterase , Heavy metals , IC50
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture > SH151-179 Fish culture > SH171-179 Diseases and adverse factors
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	20 Sep 2021 08:59
Last Modified:	20 Sep 2021 08:59
URI:	https://eprints.ums.edu.my/id/eprint/29034

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