GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis

Samina Khan Yusufzai and Mohammad Shaheen Khan and Eunice Lua Hanry and Mohd. Rafatullah and Belsheera Elison (2019) GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis. Sains Malaysiana, 48 (5). pp. 999-1009. ISSN 0126-6039 (P-ISSN) , 2735-0118 (E-ISSN)

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Abstract

In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-MS) analysis consuming hexane, chloroform and ethyl acetate as extraction solvents and gallic acid and quercetin as internal standards. In vitro antioxidant activity (AA) was determined by the application of 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay using tert-butyl-1-hydroxytoluene (BHT) as comparative drug. The GC-MS profiling showed the presence of 1-dodecanol (40.917%), oxalic acid, cyclobutyl octadecyl ester (24.985%), 1-octanol,2-nitro (12.424%), benzaldehyde, 2,4-dimethyl- (9.583%), 4-tridecanol (6.359%) and nitric acid, nonyl ester (5.616%) as major constituents. The TPC (224.62 ± 0.08 mg QE/g) and TFC (160.48 ± 0.08 mg GAE/g) was reported highest for the most polar solvent i.e. ethyl acetate. The in vitro antioxidant study disclosed highest IC50 value for ethyl acetate (2.15 mg/mL), queued by chloroform (1.19 mg/mL) and hexane (0.89 mg/mL), displaying that polar solvents are good extraction solvents for the identification of free radical scavenging properties, TFC and TPC.

Item Type:	Article
Keyword:	Antioxidant activity , GC-MS , Medicinal plants , Total flavonoid content , Total phenolic content
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QK Botany > QK1-989 Botany > QK710-899 Plant physiology
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	26 Oct 2021 16:04
Last Modified:	26 Oct 2021 16:04
URI:	https://eprints.ums.edu.my/id/eprint/30738

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