Evaluation of clonal uniformity in class one Malaysia commercial cocoa clones based on SSR markers

Lea Johnsiul and Azwan Awang (2019) Evaluation of clonal uniformity in class one Malaysia commercial cocoa clones based on SSR markers. International Journal of Agriculture, Forestry and Plantation, 8. pp. 12-17. ISSN 2462-1757

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URL: https://www.researchgate.net/publication/338478221...

Abstract

The Malaysian Cocoa Board has shortlisted 54 cocoa clones to be the recommended commercial clones distributed for farmers’ planting. These 54 cocoa clones are divided into 4 classes, Class I, Class II, Class III and Class IV according to their adaptability to a wide range of Malaysia agro-climatic condition, good agronomic traits, tolerant to major pests and diseases and high butter fat content and good flavour. Propagation of cocoa plants is usually carried out vegetatively and therefore should be genetically uniform as genetic uniformity in cocoa clones is a very crucial information for germplasm conservation, in obtaining correct parental crosses in breeding program and plant materials generation for farmers’ fields’ distribution. The evaluation was done using microsatellite markers aimed to determine the genetic uniformity of five Class I Malaysian Commercial Cocoa clones using the International Cocoa Molecular Markers Standard. Comparison were done between samples taken from one cocoa germplasm collection in Kota Kinabalu, Sabah. Two samples were taken randomly to represent each of the clones. DNA extraction was done using Qiagen DNeasy Plant DNA kit and amplification was done using 14 SSR markers. The polymorphic information content (PIC) analysis was carried out and was very informative in identifying the genetic uniformity of the evaluated cocoa samples. Result showed that there are differences between the same clone name but obtained from the same location indicating there are off-type plants in the collection.

Item Type:	Article
Keyword:	Genetic uniformity , Microsatellite markers , SSR markers , Theobroma cacao L. , Malaysian commercial cocoa clones
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK710-899 Plant physiology S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	17 Nov 2021 15:17
Last Modified:	17 Nov 2021 15:17
URI:	https://eprints.ums.edu.my/id/eprint/30945

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