Extraction and physicochemical properties of refined kappa-carrageenan from Kappaphycus Alvarezii originated from Semporna, Sabah

Adnin Awalludin and Nazirah Mingu and Abu Zahrim Yaser and Hasmadi Mamat and Khairul Azfar Kamaruzaman and Zuhair Jamain and Md Lutfor Rahman and Mohd Hafiz Abd Majid and Mohd Sani Sarjadi (2021) Extraction and physicochemical properties of refined kappa-carrageenan from Kappaphycus Alvarezii originated from Semporna, Sabah. Journal of Applied Science and Engineering, 25. pp. 411-416. ISSN 2708-9975 (E-ISSN) , 2708-9967 (P-ISSN)

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Abstract

In this research, the influence of different solvents (1.0 M KOH and 1.0 M NaOH) and different drying methods (freeze dryer and oven) towards the chemical and physical properties of refined carrageenan by using kappaphycus alvarezii collected from Semporna, Sabah were determined. The physical characterizations involved were viscosity, surface morphology and functional group while, the chemical characterization was sulphate content. The result showed that the carrageenan yield were 12% (carrageenan extracted using 1.0 M KOH) and 18% (carrageenan extracted using 1.0 M NaOH) respectively for oven as a drying method. The carrageenan yield using freeze dryer were 10% (carrageenan extracted using 1.0 M KOH) and 12% (carrageenan extracted using 1.0 M NaOH). The functional groups identification for sulphate ester, anhydro-galactose and galactose were analyzed by using FT-IR. The surface morphology of carrageenan was analyzed by using SEM for different drying method and carrageenan extracted using 1.0 M KOH (Oven as drying method) showed a smooth surface compared with other sample. The sulphate content was analyzed by using UV-Vis spectrophotometer. The concentration of sulphate were 22.5 mg/L (carrageenan extracted using 1.0 M KOH) and 69.4mg/L (carrageenan extracted using 1.0 M NaOH) for oven as drying method. The result for freeze dryer method were 33.8 mg/L (carrageenan extracted using KOH) and 44.9 mg/L (carrageenan extracted using NaOH), respectively.

Item Type: Article
Keyword: Extraction , Refined kappa-carrageenan , Kappaphycus alvarezii
Subjects: Q Science > QK Botany > QK1-989 Botany > QK504-(638) Cryptogams
T Technology > TX Home economics > TX1-1110 Home economics > TX341-641 Nutrition. Foods and food supply
Department: FACULTY > Faculty of Science and Natural Resources
Depositing User: SITI AZIZAH BINTI IDRIS -
Date Deposited: 13 Dec 2021 14:58
Last Modified: 13 Dec 2021 14:58
URI: https://eprints.ums.edu.my/id/eprint/31389

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