Detection of African swine fever virus in Sabah by loop mediated isothermal amplification (lamp) assay

Ainol Azifa Mohd Faik and Aaron Chung Yin Fung and Suraya Abdul Sani and Mohd Khalizan Sabullah and Nieccorita Wong and Ag. Muhammad Sagaf (2021) Detection of African swine fever virus in Sabah by loop mediated isothermal amplification (lamp) assay.

	Text Detection of African swine fever virus in Sabah by loop mediated isothermal amplification (lamp) assay.ABSTRACT.pdf Download (62kB)
	Text DETECTION OF AFRICAN SWINE FEVER VIRUS IN SABAH BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ASSAY.pdf Restricted to Registered users only Download (168kB) \| Request a copy

Official URL: https://drive.google977-2-73-522200-2.com/file/d/1...

Abstract

African swine fever (ASF) is a highly contagious disease that can cause severe illness and a high mortality rate in pigs. It is caused by African swine fever virus (ASFV), from the genus Asfivirus and family Asfarviridae. This disease has caused devastating outbreaks in various regions across the world. The first ASF outbreak in Malaysia was reported on February 2021, that affected three districts in Sabah: Kudat, Kota Marudu and Beluran. This virus has since spread to other district across Sabah infecting domestic and wild bearded pigs. These outbreaks are often difficult to control due to the lack of access to molecular laboratories. A fast and sensitive point-of- care (POC) test is needed to detect the presence of ASFV. This paper presents the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay to aid in the detection of ASFV samples. The performance of this assay was assessed in 10 clinical ASFV samples confirmed by two veterinary diagnostic laboratories. LAMP was also evaluated and in agreement with end point PCR. The removal of DNA extraction steps provides an extra advantage for rapid detection of ASFV.

Item Type:	Proceedings
Keyword:	ASFV , Colorimetric , LAMP , Endpoint PCR , Sabah
Subjects:	S Agriculture > SF Animal culture > SF1-1100 Animal culture > SF600-1100 Veterinary medicine > SF951-997.5 Diseases of special classes of animals
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	31 Mar 2022 11:12
Last Modified:	31 Mar 2022 11:12
URI:	https://eprints.ums.edu.my/id/eprint/32123

Actions (login required)

View Item