Preparation and characterisation of cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane

Suryani Saallah and M. Nazli Naim and Mohd Noriznan Mokhtar and Noor Fitrah Abu Bakar and Masao Gen and Lenggoro, I. Wuled (2021) Preparation and characterisation of cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane. Journal of Fiber Science and Technology, 73. pp. 251-260. ISSN 0037-9875

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URL: https://www.jstage.jst.go.jp/article/fiberst/73/10...

Abstract

An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentration ranging from 1.5 to 7.5 % to the PVA solution (8 wt%) caused significant changes to the liquid jet behaviours which consequently affected the nanofiber structures and sizes. Incorporation of CGTase in the PVA membrane was confirmed by Raman spectroscopic analysis. The Raman spectra also showed no structural changes occured to the enzyme after subjected to the electrostatic spinning and cross-linking reaction. The immobilised enzyme showed excellent catalytic efficiency with up to 3.6 times higher enzyme loading, 25 % higher activity and good reusability in comparison with CGTase/PVA film made up from the same starting solution (control).

Item Type:	Article
Keyword:	Crosslinking , Cyclodextrins , Nanofibers
Subjects:	T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP1080-1185 Polymers and polymer manufacture
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	18 May 2022 14:30
Last Modified:	18 May 2022 14:30
URI:	https://eprints.ums.edu.my/id/eprint/32594

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