The isolation rate of culturable actinomycetes from Malaysian Borneo forests and their activity against mammalian GSK-3β

Fauze Mahmud and Salahaudin Maili and Dg. Nur Azierah Fachyuni Abdul Aziz and Levianita Marajin and Mary Jembun and Siew, Eng How and Jualang @ Azlan Abdullah Gansau and Hasidah Mohd. Sidek and Noor Embi and Lee, Ping Chin (2022) The isolation rate of culturable actinomycetes from Malaysian Borneo forests and their activity against mammalian GSK-3β. Current Applied Science and Technology, 22. pp. 1-14. ISSN 2586-9396

	Text The isolation rate of culturable actinomycetes from Malaysian Borneo forests and their activity against mammalian GSK-3β.pdf Restricted to Registered users only Download (459kB) | Request a copy
	Text The isolation rate of culturable actinomycetes from Malaysian Borneo forests and their activity against mammalian GSK-3β _ABSTRACT.pdf Download (101kB)

Abstract

More than ten types of forests can be found in Sabah, Malaysian Borneo. Studies comparing culturable actinomycetes potential in this region are relatively scarce. This study described a preliminary statistical comparison of culturable actinomycetes isolation rates and their biological activity against mammalian glycogen synthase kinase-3 (GSK-3β). We isolated 1049 isolates using standard isolation media for actinomycetes (HVA, ISP4 and AIA) with distinctive morphologies from the main forest types in Sabah; primary, secondary, mangrove, and beach forests. Isolate prevalence analysis revealed that secondary forests had the highest soil-to-isolate ratio (1:11). Interestingly, Kruskal-Wallis analysis revealed no significant differences in the overall isolation rates of actinomycetes, including non-sporulating strains, between forest types (P-value=0.142). The crude extracts of these isolates were assayed against GSK-3β, and we identified 19 active isolates; nine from primary and nine from secondary forests (no significant mean difference (P-value=0.558), one from beach forests, and none from mangrove forests. Overall, despite the different sampling locations and soil types, the isolation rates of culturable actinomycetes in Sabah did not vary significantly. However, both primary and secondary forests yielded more actinomycetes isolates that were active against mammalian GSK-3β than mangrove and beach forests. Hence, secondary forests are an attractive alternative to primary forests for exploring bioactive compounds from culturable actinomycetes in Sabah.

Item Type:	Article
Keyword:	Actinomycetes , Sabah rainforests , Statistical comparison , Isolation rate , GSK-3β inhibitor
Subjects:	S Agriculture > SD Forestry > SD1-669.5 Forestry
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	14 Jul 2022 18:48
Last Modified:	14 Jul 2022 18:48
URI:	https://eprints.ums.edu.my/id/eprint/33202

Actions (login required)

View Item