Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer

Zainul Amirudin Zakaria and Nur Liana Md Nasir and Noorsyaza Eddrina Kamsani and Huzwah Khaza’ai and Lilis Sulistyorini and Roro Azizah (2022) Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer. Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas, 21. pp. 654-670. ISSN 0717-7917

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Abstract

Methanol extract of Muntingia calabura L. leaf (MEMCL) has been shown to exert the antiproliferative activity against the HT-29 (human colon adenocarcinoma) cell line. To further investigate on the medicinal potential of this plant, MEMCL was sequentially partitioned to obtain the petroleum ether, ethyl acetate and aqueous partitions, which was then tested against the HT-29 cell line and also subjected to the in vitro anti-inflammatory study. The most effective partition was also subjected to the phytoconstituents analysis using the UHPLC-ESI-MS. Findings showed that the ethyl acetate partition (EAP) exerts the most effective antiproliferative activity (IC50 = 58.0 ± 12.9 µg/mL) without affecting the 3T3 normal fibroblast cells, exhibits the highest anti-inflammatory effect when assessed using the lipoxygenase (> 95%) and xanthine oxidase (> 70%) assays, and contained various types of polyphenolics. In conclusion, M. calabura exerts apoptotic-mediated antiproliferative activity, partly via the anti-inflammatory action and synergistic action between the polyphenolics.

Item Type:	Article
Keyword:	Muntingia calabura , Ethyl acetate partition of methanol extract , HT-29 cells , Antiproliferative activity , Flavonoids
Subjects:	R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens R Medicine > RV Botanic, Thomsonian, and eclectic medicine > RV1-431 Botanic, Thomsonian, and eclectic medicine
Department:	FACULTY > Faculty of Medicine and Health Sciences
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	16 Jul 2022 18:39
Last Modified:	16 Jul 2022 18:39
URI:	https://eprints.ums.edu.my/id/eprint/33276

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