Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots

Affendy Hassan and Dorte Bodin Dresbølla and Camilla Ruø Rasmussen and Annemette Lyhne-Kjærbye and Mette Haubjerg Nicolaisen and Michaela Schiller Stokholm and Ole Søgaard Lund and Kristian Thorup-Kristensen (2019) Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots. Archives of Agronomy And Soil Science, 67 (1). pp. 15-28. ISSN 0365-0340

	Text Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots.pdf Download (40kB)
	Text Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots1.pdf Restricted to Registered users only Download (1MB)

URL: https://www.tandfonline.com/doi/full/10.1080/03650...

Abstract

This study was performed to i) compare root distribution of legume/nonlegume intercrops determined by qPCR and amplicon sequencing (Metabarcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying root growth of individual species grown in mixtures. Red beet (Beta vulgaris L. cv Detroit) and lucerne (Medicago sativa L. cv Creno) were grown in one-meter-high transparent tubes. Root competition was studied by direct observation on the tube surface determining root intensity (RI), root wash and extraction followed by determination of root length density (RLD), and DNA-based methods. Intercropping decreased lucerne RI strongly, whereas the RI of red beet was not affected. The determination of RLD did not allow us to distinguish species in mixed samples. However, both DNA methods showed apparently a tendency to overestimate the fraction of lucerne roots compared to the direct root observations. The discrepancy between methods was explained by the fact that visual methods provide estimates of root length while the DNA methods provide estimates of root mass. In conclusion, DNA-based estimates of species fractions in mixed root samples give valuable information on root interactions in mixed crops.

Item Type:	Article
Keyword:	Rhizotron , Root growth , qPCR technique , Amplicon sequencing , Intercropping
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK640-(707) Plant anatomy
Department:	FACULTY > Faculty of Tropical Forestry
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	29 Aug 2022 10:59
Last Modified:	29 Aug 2022 10:59
URI:	https://eprints.ums.edu.my/id/eprint/34021

Actions (login required)

View Item