Isolation and characterization of werneria chromene and dihydroxyacidissimol from burkillanthus malaccensis (Ridl.) swingle

Masyitah Zulkipli and Nuzum Mahbub and Ayesha Fatima and Stefanie Lim Wan-Lin and Teng-Jin Khoo and Tooba Mahboob and Mogana Rajagopal and Chandramathi Samudi and Gheetanjali Kathirvalu and Nor Hayati Abdullah and Ana Rita Pinho and Sonia M. R. Oliveira and Maria de Lourdes Pereira and Mohammed Rahmatullah and Anamul Hasan and Alok K. Paul and Mark S. Butler and Muhammad Nawaz and Polrat Wilairatana and Veeranoot Nissapatorn and Christophe Wiart (2022) Isolation and characterization of werneria chromene and dihydroxyacidissimol from burkillanthus malaccensis (Ridl.) swingle. Plants, 11 (1388). pp. 1-21. ISSN 2223-7747

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URL: https://www.mdpi.com/2223-7747/11/11/1388/pdf

Abstract

The secondary metabolites of endemic plants from the Rutaceae family, such as Burkillanthus malaccensis (Ridl.) Swingle from the rainforest of Malaysia, has not been studied. Burkillanthus malaccensis (Ridl.) Swingle may produce antibacterial and antibiotic-potentiating secondary metabolites. Hexane, chloroform, and methanol extracts of leaves, bark, wood, pericarps, and endocarps were tested against bacteria by broth microdilution assay and their antibiotic-potentiating activities. Chromatographic separations of hexane extracts of seeds were conducted to investigate effective phytochemicals and their antibacterial activities. Molecular docking studies of werneria chromene and dihydroxyacidissiminol against SARS-CoV-2 virus infection were conducted using AutoDock Vina. The methanol extract of bark inhibited the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa with the minimum inhibitory concentration of 250, 500, and 250 µg/mL, respectively. The chloroform extract of endocarps potentiated the activity of imipenem against imipenem-resistant Acinetobacter baumannii. The hexane extract of seeds increased the sensitivity of P. aeruginosa against ciprofloxacin and levofloxacin. The hexane extract of seeds and chloroform extract of endocarps were chromatographed, yielding werneria chromene and dihydroxyacidissiminol. Werneria chromene was bacteriostatic for P. aeruginosa and P. putida, with MIC/MBC values of 1000 > 1000 µg/mL. Dihydroxyacidissiminol showed the predicted binding energies of -8.1, -7.6, -7.0, and -7.5 kcal/mol with cathepsin L, nsp13 helicase, SARS-CoV-2 main protease, and SARS-CoV-2 spike protein receptor-binding domain S-RBD. Burkillanthus malaccensis (Ridl.) Swingle can be a potential source of natural products with antibiotic-potentiating activity and that are anti-SARS-CoV-2.

Item Type:	Article
Keyword:	Antibiotic potentiator , Burkillanthus malaccensis , Cathepsin L , Dihydroxyacidissiminol , Nsp13 helicase , SARS-CoV-2 , Spike protein , Werneria chromene
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK710-899 Plant physiology
Department:	INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	22 Sep 2022 10:58
Last Modified:	22 Sep 2022 10:58
URI:	https://eprints.ums.edu.my/id/eprint/34159

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