Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator

Md. Shafiquzzaman Siddiquee and Nor Azah Yusof and Abu Bakar Salleh and Tan, Soon Guan and Fatimah Abu Bakar (2011) Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator. Microchimica Acta, 172 (4). pp. 357-363. ISSN 0026-3672

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URL: https://link.springer.com/content/pdf/10.1007/s006...

Abstract

An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10–14 to 1.8 × 10–4 mol L-1, with a detection limit of 1.0 × 10–15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms.

Item Type:	Article
Keyword:	DNA electrochemical biosensor , DNA hybridization , ZnO nanopaticles , Ionic liquid , Chitosan , Trichoderma harzianum
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QR Microbiology > QR1-502 Microbiology
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	27 Sep 2022 10:53
Last Modified:	27 Sep 2022 10:53
URI:	https://eprints.ums.edu.my/id/eprint/34164

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