Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane

Suryani Saallah and Jumardi Roslan and Flavian Sheryl Julius and Sharinee Saallah and Umi Hartina Mohamad Razali and Wolyna Pindi and Mohd Rosni Sulaiman and Khairul Faizal Pa’ee and Siti Mazlina Mustapa Kamal (2021) Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane. Molecules, 26 (2564). pp. 1-11. ISSN 1420-3049

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URL: https://www.mdpi.com/1420-3049/26/9/2564/htm

Abstract

Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.

Item Type:	Article
Keyword:	Sea cucumber , Collagen , Dialysis , Ultrafiltration membrane , Physicochemical properties
Subjects:	T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP248.13-248.65 Biotechnology
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	20 Oct 2022 09:18
Last Modified:	20 Oct 2022 09:18
URI:	https://eprints.ums.edu.my/id/eprint/34506

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