Development of nested polymerase chain reaction for detection of rabies

Fazilah Muhamad Alwi and Musherah Khusaini and Ag. Muhammad Sagaf Abu Bakar and Ainol Azifa Mohd Faik (2022) Development of nested polymerase chain reaction for detection of rabies.

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Abstract

Rabies is an acute progressive and zoonotic disease which is caused by the rabies virus. Rabies virus is the prototype virus belonging to the genus lyssavirus in the family Rhabdoviridae of the order Mononegavirales. The aim of this study was to develop a nested reverse-transcriptase polymerase chain reaction (nRTPCR) for the detection of this virus along with a canine internal control to monitor the integrity of the extracted RNA. A pair of outer and inner primer targeting the N gene of the virus was evaluated along with β-actin gene as the internal control. This nRTPCR can detect up to 20 viral copy number.

Item Type:	Proceedings
Keyword:	Nested reverse-transcriptase polymerase chain reaction , (nRTPCR) , β-actin , Internal control , Limit of detection , Copy number
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR355-502 Virology
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	31 Oct 2022 10:03
Last Modified:	31 Oct 2022 10:03
URI:	https://eprints.ums.edu.my/id/eprint/34517

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