Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination

S A M Khalidi and Mohd Khalizan Sabullah and Jualang @Azlan Abdullah Gansau and Ainol Azifa Mohd Faik and S A Sani and Roslina Jawan and D D M Hamdan and M Y Shukor (2021) Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination. In: 14th Seminar on Science and Technology 2021 (S&T 2021), 8 - 9 September 2021, Virtually.

	Text Abstract.pdf Download (41kB)
	Text Full text.pdf Restricted to Registered users only Download (483kB) \| Request a copy

Official URL: https://iopscience.iop.org/article/10.1088/1742-65...

Abstract

Insecticides are an example of an environmental contaminant that can cause harmful effects on various types of organisms. Implementation of continuous monitoring program is needed to ensure the level of contamination can be controlled. In this present study, acetylcholinesterase (AChE) from the brain of Monopterus albus was elucidated to determine the potential alternative source of biosensor kit, which is sensitive towards various insecticides, especially carbamates. AChE from M. albus brain was extracted and purified through ammonium sulfate precipitation followed by procainamide-based affinity chromatography. Carbamate insecticides were used, such as bendiocarb, carbaryl, carbofuran, methomyl, and propoxur, to test their ability to inhibit AChE activity. Bendiocarb and methomyl show the capability to inhibit almost half of the enzyme activity at 51.05 and 51.20 %, respectively, while carbaryl, propoxur, and carbofuran inhibit 43.03, 42.80, and 15.06 %, respectively. Bendiocarb and methomyl were selected, and M. albus AChE was separately exposed with different concentrations of those carbamates and half maximal inhibitory concentration; IC50 was determined at 0.874 and 1.639 ppm, respectively. A field trial was conducted by testing the enzyme with various vegetable samples. All samples show no significant effect on AChE activity, meaning there was no existence of insecticides in each sample (p > 0.05). This study could be used as an alternative source for developing biosensor kits for the environmental monitoring program.

Item Type:	Conference or Workshop Item (Paper)
Keyword:	Acetylcholinesterase , Monopterus albus , Carbamates contamination
Subjects:	Q Science > QH Natural history > QH1-278.5 Natural history (General) > QH1-(199.5) General Including nature conservation, geographical distribution
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	31 Oct 2022 11:00
Last Modified:	31 Oct 2022 11:00
URI:	https://eprints.ums.edu.my/id/eprint/34618

Actions (login required)

View Item