Biological and physical characterization of bacteriophage JHA against multidrug-resistant Acinetobacter baumannii

Heba Ajmal and Zubaida Sharif and Basit Zeshan and Nureen Zahra and Madiha Khan (2022) Biological and physical characterization of bacteriophage JHA against multidrug-resistant Acinetobacter baumannii. Pakistan Journal of Pharmaceutical Sciences, 35 (5). pp. 1327-1331. ISSN 1011-601X

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Abstract

Due to the emergence of antibiotic resistance, bacteriophage therapy appears to be an ideal weapon to utilize against pathogenic bacteria. This study aimed to isolate, identify and characterize the lytic bacteriophage effective against the multidrug-resistant Acinetobacter baumannii clinical isolates. The isolated bacteriophage caused lysis by applying the double-layer agar technique on A. baumannii up to 99% in 18 hours of incubation at 37ºC. The bacterial growth reduction assay exhibited that JHA phage had high adsorption rates and could rapidly inhibit bacterial growth. The pH and thermal stability testing showed that JHA phage was stable in vast ranges of pH from 5 to 9 but its activity was highest at pH7 (1860000±1000 pfu/mL). It was stable in broad ranges of temperatures from 25ºC to 60ºC but the highest activity was found at 37ºC (1300000±30000 pfu/mL). One-step growth test results showed that it has a short latent period, strong lytic ability, high burst size, and adsorption rates and was host specific. Scanning electron microscopy (SEM) of JHA phage demonstrated icosahedral heads and tailless particles. Transmission electron microscopy (TEM) revealed JHA phage belongs to Tectiviridae family. All the characteristics of JHA phage possess lytic activity against A. baumannii strains and exhibit novel candidates to use as an alternative competitor to antibiotics in controlling such infections.

Item Type:	Article
Keyword:	Tectiviridae , Lytic bacteriophage , MDR , Scanning EM , Transmission EM , Acinetobacter baumannii
Subjects:	S Agriculture > SF Animal culture > SF1-1100 Animal culture > SF600-1100 Veterinary medicine > SF781-809 Communicable diseases of animals (General)
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	10 Nov 2022 20:25
Last Modified:	10 Nov 2022 20:25
URI:	https://eprints.ums.edu.my/id/eprint/34795

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