A NADC30-like PRRSV causes serious intestinal infections and tropism in piglets

Xin Ma and PeiXin Wang and RiTeng Zhang and Yongxin Zhao and Yifan Wu and Chen Luo and Basit Zeshan and Zengqi Yang and Li Qiu and Yefei Zhou and Xinglong Wang (2022) A NADC30-like PRRSV causes serious intestinal infections and tropism in piglets. Veterinary Microbiology, 268. p. 1. ISSN 0378-1135

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to China's swine industry. Currently, a novel type 2 PRRSV, called the NADC30-like strain, is epidemic in numerous provinces of China. In this study, a NADC30-Like PRRSV strain was isolated in primary alveolar macrophage (PAM) cells from fecal samples collected from a local pig farm, which suffered severe diarrhea. A pathogenicity comparison study was conducted in 6–week‐old piglets by inoculating highly pathogenic HP-PRRSV and NADC30-Like PRRSV isolates. RT-qPCR revealed detection of NADC30-Like PRRSV but not the HP-PRRSV in the intestine. PRRSV infection-related lesions were observed in the intestine were further confirmed by histopathological and immunohistochemically examination (IHC). In addition, severe virus infections were also detected by RT-qPCR. Based on clinical observation and pathogenicity experiments, we confirmed that NADC30-Like PRRSV gained more tissue tropism, especially in the small intestine. This may be the one reason explaining why NADC-Like 30 PRRSV become a major epidemic strain in China since the first outbreak in 2013.

Item Type:	Article
Keyword:	PRRSV , NADC30-like , Pathogenicity , Intestinal
Subjects:	S Agriculture > SF Animal culture > SF1-1100 Animal culture > SF600-1100 Veterinary medicine > SF781-809 Communicable diseases of animals (General)
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	10 Nov 2022 20:35
Last Modified:	10 Nov 2022 20:35
URI:	https://eprints.ums.edu.my/id/eprint/34800

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