Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children

Tehreem Ali and Arslan Sarwar and Aftab Ahmad Anjum and Tahir Yaqub and Basit Zeshan and Muhammad Asad Ali and Mian Muhammad Khubaib Sattar (2022) Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children. Pakistan Journal of Pharmaceutical Sciences, 35. pp. 1-9. ISSN 1011-601X

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Abstract

Activity of plant essential oils and their fractions was evaluated against characterized isolates of antibiotic resistant Enterococcus faecalis recovered from diarrheic children. The isolates were confirmed by polymerase chain reaction (PCR) targeting 16S rRNA gene amplification followed by nucleotide sequencing and accession numbers retrieved were MW349990.1, MW349859.1, MW332122.1, MW356805.1, MW349975.1, MW349988.1, MW356790.1, MW356244.1, MW341593.1 and MW332549.1. These isolates were screened for antibiotic susceptibility to a wide range of antibiotic groups and mean zone of inhibition (ZOI) of all antibiotics were recorded. Antibacterial activity of plant essential oils (n=05) was checked against three antibiotic resistant isolates of E. faecalis. Three plant essential oils having higher ZOI including Cinnamomum verum, Syzygium aromaticum and Nigella sativa were used against resistant E. faecalis isolates to determine minimum inhibitory concentration (MIC). The lowest MIC observed was of S. aromaticum (11.39±3.94 mg mL-1). The S. aromaticum n-hexane plus chloroform fraction displayed higher mean ZOI (16.67±2.51 mm), while the lowest MIC was of n-hexane oil fraction. Based upon gas chromatography-mass spectrometry (GC/MS) analysis, the most effective fatty acid was eugenic acid which is present in higher proportion in both fractions. These fractions of essential oils proved safe for the treatment of antibiotic resistant diarrheic cases of children caused by E. faecalis.

Item Type:	Article
Keyword:	Enterococcus faecalis , Antibiotic resistance , Syzygium aromaticum , Eugenic acid , GC/MS
Subjects:	R Medicine > RJ Pediatrics > RJ1-570 Pediatrics > RJ101-103 Child health. Child health services S Agriculture > SB Plant culture > SB1-1110 Plant culture
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	10 Nov 2022 20:37
Last Modified:	10 Nov 2022 20:37
URI:	https://eprints.ums.edu.my/id/eprint/34801

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