Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers

Yew, Swe Shyan and Abdul Shukor Juraimi and M.Y. Rafii and M. Shabanimofrad and Md. Amirul Alam and Md. Kamal Uddin and M. A. Hakim (2014) Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers. Life Science Journal, 11. pp. 425-430. ISSN 1097-8135 (P-ISSN) , 2372-613X (E-ISSN)

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Abstract

Bermudagrass is a widely distributed turf grasses use in Malaysia. Nevertheless, the genetic variation on molecular level of bermudagrass was insufficient. In this study, a sum of 15 cultivars were collected from different accessions in Malaysia and being analyzed using Inter-simple Sequence Repeat Markers (ISSR). A total of 172 fragments were generated from 15 selected ISSR primers. There are 164 polymorphic bands with 95.3% of polymorphism. Fragment size ranged from 120 to 1867bp. The cultivars were clustered into 5 major groups at coefficient level of 0.5 through arithmetic average (UPGMA) cluster analysis of Jaccard’s similarity coefficient. The genetic similarity coefficient was range from 0.25 to 0.61 with an average of 0.43. Satiri and Melaka cultivars were clustered separately from their own hybrid and common clusters, respectively. These findings indicated that ISSR marker is an effective molecular method use to study genetic variation of bermudagrass and could be used for varietal development program.

Item Type:	Article
Keyword:	Bermudagrass , ISSR markers , Genetic diversity , Polymorphic
Subjects:	Q Science > QK Botany > QK1-989 Botany S Agriculture > SB Plant culture > SB1-1110 Plant culture
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	22 Nov 2022 11:27
Last Modified:	22 Nov 2022 11:27
URI:	https://eprints.ums.edu.my/id/eprint/34921

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