Preliminary Detection Method for Heavy Metal Biomonitoring via Inhibitive Assay of Brain Acetylcholinesterase from Diodon hystrix

Noreen Nordin and Rahmath Abdulla and Rohana Tahir and Roslina Jawan and Diana Demiyah Mohd Hamdan and Nur Zaida Zahari and Jualang Azlan Gansau and Mohd Yunus Shukor and Mohd Khalizan Sabullah (2021) Preliminary Detection Method for Heavy Metal Biomonitoring via Inhibitive Assay of Brain Acetylcholinesterase from Diodon hystrix. Journal of Sustainability Science and Management, 18 (2). pp. 1-10. ISSN NULL

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URL: https://jssm.umt.edu.my/?page_id=5923

Abstract

Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in a water sample. An inhibitive enzyme assay was conducted, and the activity of AChE is found to be sensitive (> 20% inhibition) to sub-million levels of arsenic, cadmium, nickel, and zinc, and less sensitive (< 20% inhibition) to copper and lead. While exposure to argentum, cobalt, and chromium show no significant inhibition; AChE is not sensitive to those metal ions (p < 0.05). Field test work has proved that the assay is suitable for preliminary detection of heavy metal contamination in the river, especially near industrial and mining sites. Secondary validation was performed using ICP-OES to identify and measure the number of elements in the sample and compare them to the inhibition level of AChE activity

Item Type:	Article
Keyword:	Pollution, Heavy Metal, Acetycholinesterase, Diodon hystrix
Subjects:	Q Science > QH Natural history > QH1-278.5 Natural history (General) > QH1-(199.5) General Including nature conservation, geographical distribution Q Science > QH Natural history > QH1-278.5 Natural history (General)
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	23 Aug 2023 15:32
Last Modified:	23 Aug 2023 15:32
URI:	https://eprints.ums.edu.my/id/eprint/36314

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