Genomic Analysis of a Novel Antarctic Bacterium, Cryobacterium sp. SO2 Provides Insights into its Genomic Potential for Production of Antimicrobial Compounds

Teoh, Chui Peng and Lavin, P. and González-aravena, M. and Clemente Michael Wong Vui Ling (2023) Genomic Analysis of a Novel Antarctic Bacterium, Cryobacterium sp. SO2 Provides Insights into its Genomic Potential for Production of Antimicrobial Compounds. Sains Malaysiana, 52. pp. 1955-1966. ISSN 0126-6039

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Abstract

A novel strain of Cryobacterium designated as SO2, was isolated from the Antarctic. Hence, this study was undertaken to gain further insight into the antimicrobial compounds and secondary metabolites produced by Cryobacterium sp. SO2. It was found that strain SO2 is a Gram-positive that exhibits an irregular rod shape, which formed yellow to orange pigmented colonies on semi-solid media. Strain SO2 grows at temperatures ranging from 4 to 25 ºC. It has a complete genomic size of 4.097 Mb. SO2 has a DNA G+C content of 68.43%, and genomic annotation showed that the genome contained 3,862 CDS, 10 rRNA, 55 tRNA and 1 tm-RNA. Phylogenetic and OrthoANI analysis suggested Cryobacterium sp. strains SO1, N22, TMB1-8, LW097, TMN39-1, C. zongtaii TMN-42, C. arcticum PAMC27867 and C. soli GCJ02 as its closest phylogenetic neighbour. Genome annotation shows that strain SO2 confers β-lactamase class A, cephalosporin-C deacetylases, and 27 drug-resistance or efflux coding genes, and allows resistance to ceftazidime. Functional annotation identifies 28.74% of predicted genes are of unknown functions. Genome mining indicates that there are six putative secondary metabolite gene clusters in strain SO2. They are made up of RRE-containing, terpene, beta-lactone, T3PKS, NAPAA, and 2dos. This finding shows strain SO2 harbours genes that may be involved in the production of compounds with antibacterial and antioxidant activities.

Item Type:	Article
Keyword:	Complete genome, Cryobacterium sp., Drug-resistant, Psychrotolerant, Secondary metabolite gene cluster
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	31 Jan 2024 15:17
Last Modified:	31 Jan 2024 15:17
URI:	https://eprints.ums.edu.my/id/eprint/38063

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