Mohamad Asrol Kalam (2018) Effect of vitamin E and C on semen characteristics, egg fertility and hatchability in village roosters. Masters thesis, Universiti Malaysia Sabah.
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Abstract
The aims of this project were to study effect of vitamin E and C on semen production characteristics and fertility in village roosters. This study was conducted from February 2016 to February 2017 at the Faculty of Sustainable Agriculture (FSA), University Malaysia Sabah. First objective of the experiment was to determine the effect of vitamin E or vitamin C supplementation (in vivo) on semen quantity and quality of village roosters. Semen characteristics (sperm gross motility, percentage of live sperm and colour score) after week 4 of supplementation with vitamin E were significantly higher (6.20 ± 0.49, 81.68 ± 2.41 and 2.80 ± 0.13, respectively) in T2 group (P <0.05). Percentages of abnormal tail spermatozoa were significantly (P<0.05) lower in (3.84 ± 1.14) T2 group after supplementation of vitamin E. Gross motility (4.00 ± 0.00) was significantly (P<0.05) higher in group supplement with 4 mg/L vitamin C compared to control groups. Percentage of abnormal head (3.00 ± 1.04) in group that supplemented with 4 mg/L vitamin C was significantly (P <0.05) lower than TO (0 mg/L) and Tl (2 mg/L) groups. From this study, it can be concluded that higher supplementation of dietary vitamin E (2 IU) or vitamin C ( 4 mg/L) is beneficial in improving the semen characteristics of village roosters after 4 weeks of supplementation. Second objective was to determine the effect of addition of vitamin E or vitamin C in semen diluent (in vitro) on sperm characteristics undergoing the chilling process in the village roosters. According to the results of this study we conclude that, the most excellent level of vitamin E for addition to the semen diluent of rooster in order to improve the sperm motility and viability and also to reduce the sperm abnormalities of the sperm up to 36 hours cooling time at 5 °C is 2 IU. The most excellent level of vitamin C for addition to the semen diluent of rooster to improve the sperm motility and viability and also to reduce the sperm abnormalities up to 24 hours cooling time at 5 °C is 1 % (w/v). Third objective was to determine the effect of vitamin E or vitamin C supplementation (in viva) on fertility and hatchability after artificial insemination. Fertility and hatchability were increase when the village roosters were supplemented with vitamin E (200 and 400 IU) in their diet in 4 weeks and only fertility was improved the village roosters supplemented with 2 mg/L of vitamin C in the drinking water. The last objective was to determine the effect of addition of vitamin E or vitamin C in semen diluent (in vitro) on fertility and hatchability after artificial insemination. Fertility was improved when the semen of village roosters added with 1 IU of vitamin E. However, fertility and hatchability were not improved when the semen of village roosters was added with vitamin C.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Vitamin C and E, Roosters, Semen, Characteristics, Fertility |
| Subjects: | Q Science > QL Zoology > QL1-991 Zoology > QL951-991 Embryology |
| Department: | SCHOOL > School of Sustainable Agriculture |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 06 Mar 2024 15:40 |
| Last Modified: | 06 Mar 2024 15:40 |
| URI: | https://eprints.ums.edu.my/id/eprint/38449 |
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