In vitro regeneration of Borneo endemic orchid Vanda hastifera Rchb.f through protocorm like-bodies

Devina David and Bong, Chung Shyan and Roslina Jawan and Hartinie Marbawi and Jualang Azlan Gansau (2023) In vitro regeneration of Borneo endemic orchid Vanda hastifera Rchb.f through protocorm like-bodies. Transactions on Science and Technology, 10. pp. 175-180.

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URL: https://tost.unise.org/pdfs/vol10/no4/ToST-10x4x17...

Abstract

A protocol for in vitro regeneration of Vanda hastifera via protocorm-like bodies (PLBs) induction from the leaf section was established. In this study, young leaves from the in vitro-grown seedlings of V. hastifera were divided into apical and basal segments and cultured on Mitra basal media. The effect of individual and combinations of plant growth regulators (PGRs) including 6-benzylaminopurine (BAP), kinetin, a-naphtaleneacetic acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) at different concentrations were studied. PLBs were initiated at the cut-end surfaces after 35 days of culture via direct or indirect regeneration pathways. The addition of PGRs singly promoted the formation of (PLBs) from both leaf segments, however, the combination of 4.0mg/L of BAP + 4.0mg/L of NAA obtained the highest PLBs formation (10.45 ± 2.47) from the leaf base explant. The present finding holds significant importance as it not only promotes largescale cultivation but also contributes to the conservation of this native orchid species.

Item Type:	Article
Keyword:	Micropropagation, Leaf explant, PLBs, NAA, BAP
Subjects:	Q Science > QK Botany > QK1-989 Botany S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB403-450.87 Flowers and flower culture. Ornamental plants
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	23 Apr 2024 14:58
Last Modified:	23 Apr 2024 14:58
URI:	https://eprints.ums.edu.my/id/eprint/38564

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