Effect of Exercise Intensity on Antioxidant Enzymatic Activities in Sedentary Adults

D. Maryama Ag. Daud and Aminuddin Abdul Hamid Karim and Nabishah Mohamad and Noor Aini Abd Hamid and Wan Zurinah Wan Ngah (2006) Effect of Exercise Intensity on Antioxidant Enzymatic Activities in Sedentary Adults. Malaysian Journal of Biochemistry and Molecular Biology, 13. pp. 37-47. ISSN 1511-2616

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Abstract

This study was designed to investigate the influence of relative exercise intensity on antioxidant enzymatic activities in sedentary healthy adults after cycling at different exercise intensity (50%VO2max, 60%VO2max and 70%VO2max) for 10 minutes. Venous blood were collected pre- and immediately post-exercise from 24 sedentary healthy adults (mean age= 20.83 ± 2.32 years, VO2max =2.15 ± 0.36 liter/min, BMI= 21.01 ± 4.27 kg/m2). Erythrocyte enzymes, i.e catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured as indirect measures of reactive oxygen species (ROS) production. (Subject cycled on the cycle ergometer with the estimated workload that corresponds to the measured maximal oxygen uptake (VO2max) that assigned or given to exercise intensity at the speed of 50 to 60 revolutions per minute (rpm) for 10 minutes.) Heart rate (HR) and VO2 were monitored every minute during exercise test to ensure the subject was exercising at the relative exercise intensity given. Data were analysed with repeated measured ANOVA and paired t-tests. SOD increased significantly (p<0.05) by 25.3%, 55.8%, and 49.6% immediately post-exercise at 50%VO2max, 60%VO2max and 70%VO2max respectively, with a significant exercise intensity effect. On the other hand, 24.1% increment in CAT activity at 50%VO2max, and 38.1% and 10.0% decrement at 60%VO2max and 70%VO2max were also significantly affected by exercise intensity (p<0.05). No significant changes in GPx activity were noted. These data suggested that ROS such as superoxide anions (O2 -), hydrogen peroxide (H2O2), and hydroxyl radicals (OH-) production increased as the exercise intensity increased. The changes in SOD, CAT and GPx activity were closely related to exercise intensity. The decrement observed in CAT and GPx activity levels would indicate the presence of oxidative stress.

Item Type:	Article
Keyword:	Axercise intensity, Reactive oxygen species,ROS, Superoxide dismutase,SOD, Glutathione peroxidase, GPx, Catalase, CAT
Subjects:	G Geography. Anthropology. Recreation > GV Recreation Leisure > GV1-1860 Recreation. Leisure > GV201-555 Physical education and training > GV460-555 Gymnastics. Gymnastic exercises Including calisthenics, heavy exercises, acrobatics, etc. H Social Sciences > HQ The family. Marriage. Woman > HQ1-2044 The Family. Marriage. Women > HQ503-1064 The family. Marriage. Home > HQ799.95-799.97 Adulthood
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	12 Jun 2024 09:28
Last Modified:	12 Jun 2024 09:28
URI:	https://eprints.ums.edu.my/id/eprint/38819

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