Mohd Nazri Abdul Rahman and Amin Ismail and Azrina Azlan and Ahmad Fazli Abdul Aziz and Nor Hayati Muhammad (2024) Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts. Malaysian Journal of Medicine and Health Sciences, 20. pp. 193-202.
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Abstract
Momordica cochinchinensis (Lour) Spreng, known as gac fruit, is rich in bioactive compounds like carotenoids (β-carotene, lycopene, and lutein). This study assessed the antioxidant, cytotoxic, and anti-adipogenic properties of gac fruit extracts (GFE) from different fractions (peel, pulp, aril), using 3T3-L1 adipocytes. Method: Gac extracts’ DPPH radical scavenging was tested with 1000µg/mL dilutions. 3T3-L1 pre-adipocyte viability was measured via MTT assay. Differentiated adipocytes were treated (75, 150, 300 µg/mL) with GFE for 7 days. Inhibitory effects on adipogenesis and lipid accumulation were studied through Oil Red O staining. Triglyceride content was quantified and compared to controls. Results: IC50 values against DPPH radicals were 660µg/mL (peel), 560µg/ mL (pulp), and 820µg/mL (aril). 3T3-L1 cell viability was unaffected up to 200µg/mL. However, 200µg/mL GFE decreased viability, inhibiting growth. Gac extracts effectively reduced lipid accumulation and hindered cell differentiation dose-dependently. Pulp extract notably reduced intracellular triglycerides, surpassing aril and peel effects. Conclusion: Gac fruit extract fractions (peel, pulp, and aril) efficiently inhibited adipogenesis in 3T3-L1 cells, evidenced by lowered lipid accumulation, triglyceride content, and cell viability. This study highlights gac fruit extracts’ potential therapeutic use against obesity.
| Item Type: | Article |
|---|---|
| Keyword: | 3T3-L1 adipocytes, Cytotoxic, Anti-adipogenic, Carotenoids, Momordica cochinchinensis |
| Subjects: | S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB354-402 Fruit and fruit culture T Technology > TS Manufactures > TS1-2301 Manufactures > TS800-937 Wood technology. Lumber > TS920-937 Chemical processing of wood |
| Department: | FACULTY > Faculty of Food Science and Nutrition |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 15 Jul 2024 12:11 |
| Last Modified: | 15 Jul 2024 12:11 |
| URI: | https://eprints.ums.edu.my/id/eprint/39176 |
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