Assessment of inhibitive assay for organophosphate insecticides using acetylcholinesterase from the brain tissue of Monopterus albus

Mohd Khalizan Sabullah and Siti Aishah Muhammad Khalidi and Rahmath Abdulla and Hartinie Marbawi and Roslina Jawan and Goh, Lucky Poh Wah and Syed Umar Faruq Syed Najmuddin and Mohd Rosni Sulaiman (2023) Assessment of inhibitive assay for organophosphate insecticides using acetylcholinesterase from the brain tissue of Monopterus albus.

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Official URL: https://oer.ums.edu.my/handle/oer_source_files/278...

Abstract

Organophosphate insecticides inhibit the development of pests by blocking the metabolisation of the neurotransmitter acetylcholine and making a strong bond at the active site of acetylcholinesterase. In this present study, Monopterus albus acetylcholinesterase was tested to evaluate its sensitivity to the nerve agent used to control the development of insect pests that affect the productivity and quality of crops. The inhibition level of acetylcholinesterase was arranged in the descending order of parathion ≥ dimethoate > malathion > chlorpyrifos = diazinon > acephate > trichlorfon. Half maximal inhibitory (IC₅₀) concentrations of parathion and dimethoate were determined at 0.162 ± 0.003 and 1.509 ± 0.152 mg.L⁻¹ , respectively, showing parathion is the highest sensitivity towards AChE activity. Commercially available insecticides, namely malathion, and chlorpyrifos were used to treat C. asiatica. Each leaf was collected from a different day of treatment, and the result shows both strongly inhibiting acetylcholinesterase activity from day 0, then total 100% acetylcholinesterase was inhibited on day 10. This study proved that M. albus AChE could be used as another alternative acetylcholinesterase source to develop environmental biosensors, especially in an agricultural area.

Item Type:	Proceedings
Keyword:	Enzyme preparation, Organophosphate Insecticides, Acetylcholinesterase
Subjects:	Q Science > QH Natural history > QH301-705.5 Biology (General) > QH540-549.5 Ecology Q Science > QL Zoology > QL1-991 Zoology > QL1-355 General Including geographical distribution
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	14 Oct 2024 10:25
Last Modified:	14 Oct 2024 10:25
URI:	https://eprints.ums.edu.my/id/eprint/41315

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