Untargeted Metabolite Profiling of Wild and In Vitro Propagated Sabah Jewel Orchid Macodes limii J.J. Wood & A.L. Lamb

Devina David and Nor Azizun Rusdi and Ruzaidi Azli Mohd. Mokhtar and Lucky Poh Wah Goh and Jualang Azlan Gansau (2024) Untargeted Metabolite Profiling of Wild and In Vitro Propagated Sabah Jewel Orchid Macodes limii J.J. Wood & A.L. Lamb. Tropical Life Sciences Research, 35 (3). pp. 1-35. ISSN 0128-4541

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URL: https://ejournal.usm.my/tlsr/article/view/tlsr_vol...

Abstract

Macodes limii J.J. Wood & A.L. Lamb is a terrestrial jewel orchid native to Sabah, recognised for its sparkling golden-yellow venations, uniformly distributed on its leaves. Despite its high ornamental value, the exploration of the plant’s medicinal potential remains ambiguous. The current study was conducted to gain a fundamental understanding of the metabolite composition and regulation in M. limii plants from two different growing environments: wild and in vitro cultivation, as well as to analyse their phytochemical contents and antioxidant activity. The metabolite profiling of the M . limii plant extracts through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has tentatively identified compounds from various classes including sugars, carbohydrates, sugar alcohols, amino acids, organic acids, phenolic derivatives and lipid and lipid-like compounds. Subsequently, the multivariate statistical analysis confirmed the existence of significant metabolite variations across distinct growth environments. Notably, the leaf extract derived from wild-grown plants displayed the highest levels of total phenolic and flavonoid content, contributing significantly to its higher antioxidant activity as measured by the 2,2- diphenyl-1-picrylhydrazyl (DPPH) assay. The discovery has offered a fundamental understanding of the metabolites in M. limii jewel orchids, indicating that in vitro regenerated plants may represent a viable alternative for further investigating their therapeutic potential, thus helping to alleviate the impact on wild populations.

Item Type:	Article
Keyword:	Orchidaceae, GC-MS, LC-MS/MS, DPPH Assay, Metabolomics
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms S Agriculture > SD Forestry > SD1-669.5 Forestry > SD250-363.3 Forestry education
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	14 Oct 2024 11:04
Last Modified:	14 Oct 2024 11:04
URI:	https://eprints.ums.edu.my/id/eprint/41346

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