David Astudillo-Barraza and Rómulo Oses Pedraza and Carlos Henríquez-Cas and Clemente Michael Vui Ling Wong and José M. Pérez-Donoso and Cristina Purcarea and Heidge Fukumasu and Natalia Fierro-Vásquez and Pablo A. Pérez and Paris Lavin (2023) Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013). Fermentation, 9. pp. 1-15.
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Abstract
The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl acetate extract (EAE) fractions were purified via thin-layer chromatography and High-Performance Liquid Chromatography (HPLC), showing that orange-colored compounds displayed antimicrobial activity against pathogenic bacteria even after shock thermal treatment. The UV–VIS features of the active compounds, the TLC assay with actinomycin-D pure standard, Fourier transform infrared (FTIR) spectra and the ANTISMASH analysis support the presence of actinomycin-like compounds. We demonstrated that S. fildesensis displays antiproliferative activity against human tumor cell lines, including human breast cancer (MCF-7), prostate cancer (PC-3), colon cancer (HT-29) and non-tumoral colon epithelial cells (CoN). The half-maximal effective concentrations (EC50) ranged from 3.98 µg/mL to 0.1 µg/mL. Our results reveal that actinomycin-like compounds of S. fildesensis induced apoptosis mediated by caspase activation, decreasing the mitochondrial membrane potential and altering the cell morphology in all tumoral and non-tumoral cell lines analyzed. These findings confirm the potential of the psychrotolerant Antarctic S. fildesensis species as a promising source for obtaining potential novel anticancer compounds.
| Item Type: | Article |
|---|---|
| Keyword: | Streptomyces Fildesensis, Cytotoxic Antimicrobial, Anticancer, Apoptosis, Mitochondrial Membrane Permeability. Caspases |
| Subjects: | R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens R Medicine > RM Therapeutics. Pharmacology > RM1-950 Therapeutics. Pharmacology > RM265-267 Antibiotic therapy. Antibiotics |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 16 Dec 2024 11:25 |
| Last Modified: | 16 Dec 2024 11:25 |
| URI: | https://eprints.ums.edu.my/id/eprint/42253 |
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