Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms

Lo, Kuo-Chin and Jualang Azlan Gansau and Shih, Chia-Hung and Kao, Chien-Yuan (2022) Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms. Horticulturae, 8. pp. 1-10.

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Abstract

This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 to 8 h before sectioning with a microtome. Optimal conditions for slicing sections of 100 to 300 µm were achieved when the protocorms were embedded at 10% (w/v) agarose and dried for 4 h under laminar flow, and the hardness of the agarose block under these conditions reached 641.8 ± 9.5 g·cm−2 . The sectioned protocorms that were cultured on an MS medium supplemented with 1.2 mg·L −1 6-benzylaminopurine and 0.1 mg·L −1 α-naphthaleneacetic acid were capable of growth and differentiated through the neoformation of protocorm-like bodies (PLBs) and/or callus before subsequent regeneration into plantlets and development into healthy plants in a nursery environment. The results of this study demonstrate that microtome-based tTCL is a reliable and promising approach for mass propagation and possible virus-free propagation objectives for Phalaenopsis.

Item Type:	Article
Keyword:	Phalaenopsis, Shoot development, Protocorm, Transverse thin cell layer, tTCL
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK710-899 Plant physiology S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB403-450.87 Flowers and flower culture. Ornamental plants
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	26 Dec 2024 11:14
Last Modified:	26 Dec 2024 11:14
URI:	https://eprints.ums.edu.my/id/eprint/42389

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