Siti Nor Fatihah and Nur Hazwani Dahon and Cheng-Ann Chen (2024) Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage. Jurnal Akuakultur Indonesia, 23. pp. 1-9.
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Abstract
The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short-term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20, and -80°C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84%) after 60 min compared the calcium-free saline and Ringer’s solution. Using 5% glycine as the optimal cryoprotectant, sperm viability at -80°C exhibited robust initial viability 95.17 ± 0.92% at 6 hours, 94.11 ± 0.64% at 12 hours, and 93.31 ± 0.51% at 24 hours. However, sperm viability gradually declined to 90.84 ± 2.05% by day 7, 82.82 ± 0.41% by day 14, and continued to decrease by day 21. In a nutshell, the phosphate buffer and 5% glycine proved to be the most effective extender and cryoprotectant in this study
| Item Type: | Article |
|---|---|
| Keyword: | cooling rate, cryoprotectant, extender, Scylla tranquebarica, sperm viability |
| Subjects: | Q Science > QL Zoology > QL1-991 Zoology > QL605-739.8 Chordates. Vertebrates > QL614-639.8 Fishes S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB320-353.5 Vegetables |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | ABDULLAH BIN SABUDIN - |
| Date Deposited: | 06 Feb 2025 13:51 |
| Last Modified: | 06 Feb 2025 13:51 |
| URI: | https://eprints.ums.edu.my/id/eprint/42778 |
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