Extraction and characterization of collagen and gelatin from body wall of sea cucumbers stichopus horrens and holothuria arenicola

Noora Barzkar and Gilan Attaran-Fariman and Ali Taheri and Balu Alagar Venmathi Maran (2024) Extraction and characterization of collagen and gelatin from body wall of sea cucumbers stichopus horrens and holothuria arenicola. PeerJ. pp. 1-23. ISSN 2167-8359

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Abstract

Background: Marine invertebrates, including sponges, molluscs, jellyfish, mussels, and sea cucumbers, are abundant sources of high-quality collagen and offer advantages such as availability, ease of processing, lower inflammatory response, and good metabolic compatibility. Approximately 70% of the total protein in the body wall of sea cucumbers is collagen. Gelatin is a water-soluble protein produced from heat-denatured collagen and has various industrial applications. Methods: Pepsin-solubilized collagen was extracted from the body wall of two sea cucumber Stichopus horrens and Holothuria arenicola, species found in the Oman Sea and characterized with SDS-PAGE and amino acid composition. Then gelatin was extracted from pepsin-solubilized collagen of S. horrens and some rheological properties were measured. Results: Amino acid composition and SDS-PAGE analysis showed that the collagen from both species was type I, with one a1 chain and β chains, with molecular weights of 125 and 250 kDa, respectively. Glycine was the most abundant amino acid in the collagen from both sea cucumber species. The pepsin-soluble collagens from both species had high levels of glycine, proline, alanine, glutamic acid, and hydroxyproline. The gelatin from S. horrens had a melting point of 30 °C and displayed exceptional thermal stability, surpassing that of mammalian gelatin. Its gelling point was 5 °C, like that of cold-water fish gelatin, with a viscosity of 2.065 cp-lower than mammal gelatins. These findings suggested that collagen and gelatin from sea cucumbers could be useful in nutraceutical, pharmaceutical and cosmetic industries.

Item Type:	Article
Keyword:	Pepsin-solubilized collagen, Gelatin, Sea cucumber, Stichopus horrens, Holothuria arenicola
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QP Physiology > QP1-(981) Physiology > QP501-801 Animal biochemistry
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	18 Feb 2025 09:31
Last Modified:	18 Feb 2025 09:31
URI:	https://eprints.ums.edu.my/id/eprint/42890

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