Morphological and biochemical characterization of glyphosate- degrading bacteria isolated from agricultural soils in Kota Marudu, Sabah

Annmarie Alexander and Jualang Azlan Gansau and Mohd Yunus Abd. Shukor and Nur Adeela Yasid and Hartinie Marbawi (2024) Morphological and biochemical characterization of glyphosate- degrading bacteria isolated from agricultural soils in Kota Marudu, Sabah.

Text FULL TEXT.pdf Restricted to Registered users only Download (675kB) | Request a copy

Abstract

Glyphosate is a widely used herbicide, but its accumulation in ecosystems presents environmental and health hazards, demanding sustainable solutions. This study aimed to isolate and evaluate local soil bacteria capable of degrading glyphosate-based herbicides. Soil samples were enriched in minimal salt medium (MSM) with Roundup for seven days, and bacterial isolates were screened for tolerance to varying Roundup concentrations. Morphological and biochemical tests were conducted, followed by partial identification using ABIS online software. Biodegradation assays assessed the ability of the isolates to break down glyphosate. Three bacterial strains, KM1, KM2, and KM4, tolerated up to 6 mL/L of Roundup. Gram staining revealed all three were Gram-positive, with KM1 being rod-shaped and KM2 and KM4 cocci. Biochemical assays indicated that strain KM1 tested positive for catalase, all sugar fermentations, the Voges-Proskauer test, and the citrate test. In contrast, strains KM2 and KM4 showed positive results for catalase, the Voges-Proskauer test, and the citrate test, but they were unable to ferment sucrose while fermenting all other sugars. ABIS online software identified strain KM1 as Bacillus sp and strain KM2 and KM4 as Staphylococcus sp. Biodegradation rates after three days were 82.8%, 83.1%, and 80.1% for KM1, KM2, and KM4, respectively. Further studies are required to confirm the identification and characterization of these bacterial strains, optimize the conditions for glyphosate degradation, and evaluate their full potential for bioremediation in agricultural environments.

Item Type:	Proceedings
Keyword:	Bioremediation, Biodegradation, Glyphosate, Isolation, Pesticide
Subjects:	Q Science > QE Geology > QE1-996.5 Geology > QE1-350.62 General Including geographical divisions S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) > S622-627 Soil conservation and protection
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	17 Mar 2025 11:09
Last Modified:	17 Mar 2025 11:09
URI:	https://eprints.ums.edu.my/id/eprint/43230

Actions (login required)

View Item